LOCUS       FCK(1.3)-Halo-GF       10864 bp    DNA     circular     21-APR-2007
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|444245275|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     LTR             835..1015
                     /vntifkey="19"
                     /label=5'LTR
     misc_structure  2440..2557
                     /vntifkey="88"
                     /label=cPPT
     LTR             6669..6849
                     /vntifkey="19"
                     /label=3'LTR
     polyA_signal    6881..7105
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      7151..7579
                     /vntifkey="33"
                     /label=F1\Ori
     rep_origin      7584..7927
                     /vntifkey="33"
                     /label=SV40\Ori
     promoter        7969..8035
                     /vntifkey="30"
                     /label=EM-7
     polyA_signal    8540..8670
                     /vntifkey="25"
                     /label=SV40\Early\PolyA
     rep_origin      9053..9726
                     /vntifkey="33"
                     /label=pUC\Ori
     misc_marker     9871..10731
                     /vntifkey="22"
                     /label=AmpR
     promoter        10766..10850
                     /vntifkey="30"
                     /label=Pbla
     misc_feature    1691..1895
                     /vntifkey="21"
                     /label=RRE
     exon            3896..4782
                     /vntifkey="61"
                     /label=Halo
     misc_feature    2598..3894
                     /vntifkey="21"
                     /label=CamKII
     exon            4788..5531
                     /vntifkey="61"
                     /label=EGFP
     enhancer        5547..6144
                     /vntifkey="9"
                     /label=WPRE
BASE COUNT     2668 a      2756 c      2872 g      2568 t 
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 
     3541 tttgttcaca tccccttctc caaccccctc agtacatcac cctgggagaa caaggtccac 
     3601 ttgcttctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caacggagga 
     3661 gcaggttttg aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa 
     3721 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct 
     3781 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcaatcaagc tggttctcca 
     3841 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc 
     3901 gccaccatga ctgagaccct cccacccgtg actgaaagcg ccgtcgctct gcaagcagag 
     3961 gttacccagc gggagctgtt cgagttcgtc ctcaacgacc ccctcctggc ttctagcctc 
     4021 tacatcaaca ttgctctggc aggcctgtct atactgctgt tcgtcttcat gaccagggga 
     4081 ctcgatgacc ctagggctaa actgattgca gtgagcacaa ttctggttcc cgtggtctct 
     4141 atcgcttcct acactgggct ggcatctggt ctcacaatca gtgtcctgga aatgccagct 
     4201 ggccactttg ccgaagggag ttctgtcatg ctgggaggcg aagaggtcga tggggttgtc 
     4261 acaatgtggg gtcgctacct cacctgggct ctcagtaccc ccatgatcct gctggcactc 
     4321 ggactcctgg ccggaagtaa cgccaccaaa ctcttcactg ctattacatt cgatatcgcc 
     4381 atgtgcgtga ccgggctcgc agctgccctc accaccagca gccatctgat gagatggttt 
     4441 tggtatgcca tctcttgtgc ctgctttctg gtggtgctgt atatcctgct ggtggagtgg 
     4501 gctcaggatg ccaaggctgc agggacagcc gacatgttta atacactgaa gctgctcact 
     4561 gtggtgatgt ggctgggtta ccctatcgtt tgggcactcg gcgtggaggg aatcgcagtt 
     4621 ctgcctgttg gtgtgacaag ctggggctac tccttcctgg acattgtggc caagtatatt 
     4681 tttgcctttc tgctgctgaa ttatctgact tccaatgagt ccgtggtgtc cggctccata 
     4741 ctggacgtgc catccgccag cggcacacct gccgatgacc gaccggtcgc caccatggtg 
     4801 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 
     4861 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 
     4921 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 
     4981 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 
     5041 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 
     5101 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 
     5161 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 
     5221 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 
     5281 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 
     5341 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 
     5401 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 
     5461 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc 
     5521 cgcgactcta gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg 
     5581 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 
     5641 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 
     5701 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 
     5761 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 
     5821 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 
     5881 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 
     5941 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 
     6001 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 
     6061 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 
     6121 ctccctttgg gccgcctccc cgcatcgatt accgtcgacc tcgagaccta gaaaaacatg 
     6181 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac 
     6241 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt 
     6301 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 
     6361 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact 
     6421 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat 
     6481 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga 
     6541 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat 
     6601 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg 
     6661 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 
     6721 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 
     6781 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 
     6841 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag 
     6901 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 
     6961 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 
     7021 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 
     7081 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 
     7141 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     7201 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
     7261 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
     7321 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
     7381 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     7441 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
     7501 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
     7561 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 
     7621 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 
     7681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 
     7741 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 
     7801 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 
     7861 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 
     7921 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta 
     7981 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc 
     8041 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt 
     8101 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt 
     8161 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac 
     8221 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt 
     8281 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg 
     8341 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga 
     8401 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg 
     8461 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 
     8521 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 
     8581 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 
     8641 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 
     8701 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 
     8761 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 
     8821 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 
     8881 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 
     8941 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     9001 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     9061 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     9121 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     9181 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     9241 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     9301 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     9361 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     9421 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     9481 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     9541 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     9601 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     9661 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     9721 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     9781 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     9841 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     9901 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     9961 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
    10021 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
    10081 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
    10141 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
    10201 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
    10261 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
    10321 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
    10381 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
    10441 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
    10501 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
    10561 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
    10621 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
    10681 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
    10741 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
    10801 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
    10861 tgac 
//
