LOCUS       FCK-hChR2-GFP          10904 bp    DNA     circular     29-SEP-2007
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|458744851|
COMMENT     VNTDBDATE|458747060|
COMMENT     LSOWNER|
COMMENT     VNTNAME|FCK-hChR2-GFP|
COMMENT     VNTAUTHORNAME|Ed Boyden|
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FEATURES             Location/Qualifiers
     misc_feature    2598..3894
                     /vntifkey="21"
                     /label=CaMKII(1.3)
     exon            3907..4836
                     /vntifkey="61"
                     /label=hChR2
     exon            4855..5571
                     /vntifkey="61"
                     /label=GFP
     LTR             835..1015
                     /vntifkey="19"
                     /label=5'LTR
     LTR             6709..6889
                     /vntifkey="19"
                     /label=3'LTR
     misc_marker     1691..1895
                     /vntifkey="22"
                     /label=RRE
     misc_signal     2440..2557
                     /vntifkey="87"
                     /label=cPPT
     misc_marker     5588..6185
                     /vntifkey="22"
                     /label=WPRE
     oriT            7191..7619
                     /vntifkey="104"
                     /label=f1\Ori
     oriT            7624..7967
                     /vntifkey="104"
                     /label=SV40\Ori
     polyA_signal    6921..7145
                     /vntifkey="25"
                     /label=polyA
     oriT            9093..9766
                     /vntifkey="104"
                     /label=pUC\Ori
     misc_marker     9911..10771
                     /vntifkey="22"
                     /label=AmpR
BASE COUNT     2702 a      2730 c      2890 g      2582 t 
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 
     3541 tttgttcaca tccccttctc caaccccctc agtacatcac cctgggagaa caaggtccac 
     3601 ttgcttctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caacggagga 
     3661 gcaggttttg aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa 
     3721 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct 
     3781 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcaatcaagc tggttctcca 
     3841 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc 
     3901 gccaccatgg actatggcgg cgctttgtct gccgtcggac gcgaactttt gttcgttact 
     3961 aatcctgtgg tggtgaacgg gtccgtcctg gtccctgagg atcaatgtta ctgtgccgga 
     4021 tggattgaat ctcgcggcac gaacggcgct cagaccgcgt caaatgtcct gcagtggctt 
     4081 gcagcaggat tcagcatttt gctgctgatg ttctatgcct accaaacctg gaaatctaca 
     4141 tgcggctggg aggagatcta tgtgtgcgcc attgaaatgg ttaaggtgat tctcgagttc 
     4201 ttttttgagt ttaagaatcc ctctatgctc taccttgcca caggacaccg ggtgcagtgg 
     4261 ctgcgctatg cagagtggct gctcacttgt cctgtcatcc ttatccacct gagcaacctc 
     4321 accggcctga gcaacgacta cagcaggaga accatgggac tccttgtctc agacatcggg 
     4381 actatcgtgt ggggggctac cagcgccatg gcaaccggct atgttaaagt catcttcttt 
     4441 tgtcttggat tgtgctatgg cgcgaacaca ttttttcacg ccgccaaagc atatatcgag 
     4501 ggttatcata ctgtgccaaa gggtcggtgc cgccaggtcg tgaccggcat ggcatggctg 
     4561 tttttcgtga gctggggtat gttcccaatt ctcttcattt tggggcccga aggttttggc 
     4621 gtcctgagcg tctatggctc caccgtaggt cacacgatta ttgatctgat gagtaaaaat 
     4681 tgttgggggt tgttgggaca ctacctgcgc gtcctgatcc acgagcacat attgattcac 
     4741 ggagatatcc gcaaaaccac caaactgaac atcggcggaa cggagatcga ggtcgagact 
     4801 ctcgtcgaag acgaagccga ggccggagcc gtgccagcgg caccggtagt agcagtgagc 
     4861 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 
     4921 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 
     4981 accctgaagt tcatttgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 
     5041 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 
     5101 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 
     5161 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 
     5221 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 
     5281 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 
     5341 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 
     5401 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 
     5461 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 
     5521 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agaattcgat 
     5581 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt 
     5641 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 
     5701 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 
     5761 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 
     5821 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 
     5881 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 
     5941 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc 
     6001 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 
     6061 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 
     6121 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 
     6181 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat 
     6241 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt 
     6301 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 
     6361 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 
     6421 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac 
     6481 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca 
     6541 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct 
     6601 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc 
     6661 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg 
     6721 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 
     6781 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 
     6841 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta 
     6901 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 
     6961 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 
     7021 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 
     7081 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 
     7141 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 
     7201 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 
     7261 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 
     7321 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 
     7381 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 
     7441 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 
     7501 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 
     7561 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta 
     7621 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga 
     7681 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 
     7741 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 
     7801 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
     7861 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag 
     7921 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt 
     7981 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg 
     8041 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc 
     8101 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt 
     8161 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt 
     8221 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc 
     8281 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg 
     8341 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc 
     8401 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac 
     8461 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 
     8521 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 
     8581 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     8641 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     8701 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 
     8761 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     8821 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     8881 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     8941 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     9001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     9061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     9121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     9181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     9241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     9301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     9361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     9421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     9481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     9541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     9601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     9661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     9721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     9781 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     9841 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     9901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     9961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
    10021 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
    10081 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
    10141 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
    10201 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
    10261 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
    10321 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
    10381 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
    10441 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
    10501 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
    10561 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
    10621 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
    10681 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
    10741 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
    10801 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
    10861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac 
//
